FastQCFastQC Report
Mon 6 Jun 2016
ERR1379220.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379220.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283518
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGACCAAATCTCGTATGCC18750.6613336719361733Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACC2700.044.99964531
GAGACCG2650.044.99964527
TCGTATG2650.044.99964543
CGGACCA2700.044.99964532
ACCGGAC2750.044.18147330
CGTATGC2700.044.16631744
CTCGTAT2700.044.16631742
GACCGGA2700.044.16631729
ATCTCGT2850.042.63124540
AATCTCG2850.042.63124539
TCTCGTA2800.042.58895541
AGACCGG2900.041.1203728
CCGAGAC2900.041.1203725
ATGCCGA3000.040.4996822
TGCCGAG2900.040.34451323
AAGAGCG3100.039.193244
CGGTTCA3100.039.193249
AGAGCGG3100.039.193245
AATGCCG3050.039.09805721
GTATGCC3050.039.09805745
Produced by FastQC (version 0.11.5)