FastQCFastQC Report
Mon 6 Jun 2016
ERR1379217.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379217.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346097
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCTAAGATCTCGTATGCC14590.421558118099839Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGCT1300.043.26811630
AGACCGG1300.043.26811628
ATCTCGT1250.043.1988940
GACCGGC1350.041.66559229
TCGTATG1300.041.5373943
CTCGTAT1350.039.9989742
TCTCGTA1350.039.9989741
GAGACCG1450.038.79210727
CGGCTAA1450.038.79210732
GTATGCC1400.038.5704445
GCGGTTC1500.037.4990358
CCGGCTA1500.037.49903531
CGGTTCA1500.037.4990359
CGTATGC1450.037.24042544
CCGAGAC1500.035.99907725
AAGAGCG1600.035.1604274
AGAGCGG1600.035.1604275
AGCGGTT1600.035.1553467
GAGCGGT1600.035.1553466
AATGCCG1650.032.72643321
Produced by FastQC (version 0.11.5)