FastQCFastQC Report
Mon 6 Jun 2016
ERR1379212.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379212.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378237
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTATTGATCTCGTATGCC13170.3481943860595341Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1500.042.043
CCGCTAT1450.041.8965531
CTCGTAT1550.040.6451642
ACCGCTA1500.040.530
AGACCGC1500.040.528
CGTATGC1600.039.37544
CGCTATT1600.039.37532
TCTCGTA1600.039.37541
CGGAAGA1750.038.5714261
AGCGGTT1700.038.382357
ATCTCGT1650.038.18181640
GACCGCT1600.037.9687529
GAGACCG1650.036.8181827
GCGGTTC1800.036.258
CGGTTCA1850.035.270279
GAGCGGT1850.035.270276
AGAGCGG1900.034.3421065
AATGCCG1850.034.05405421
CCGAGAC1800.033.7525
GTATGCC1950.033.46153645
Produced by FastQC (version 0.11.5)