FastQCFastQC Report
Mon 6 Jun 2016
ERR1379209.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379209.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269476
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTGTTATCTCGTATGCC11010.40857070759548164Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGAC1150.045.00000425
AGACCGT1200.043.12500428
CTCGTAT1150.043.0434842
TATCTCG1150.043.0434839
TCTCGTA1150.043.0434841
TGCCGAG1300.041.5384623
GAGACCG1250.041.427
ATCTCGT1200.041.25000440
AAGAGCG1300.039.807694
CGTGTGT1300.039.8076932
ATGCCGA1300.039.8076922
TCGTATG1250.039.60000243
CGGTTCA1350.038.3333329
CCGTGTG1350.038.33333231
ACCGTGT1350.038.33333230
CGTATGC1300.038.0769244
GCGGTTC1400.036.9642838
GACCGTG1400.036.96428329
AATGCCG1400.036.96428321
GCCGAGA1400.036.96428324
Produced by FastQC (version 0.11.5)