FastQCFastQC Report
Mon 6 Jun 2016
ERR1379206.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379206.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394641
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTACGTTATCTCGTATGCC17660.4474953185300058Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATC1950.044.99860436
CGTACGT1950.044.99860432
CCGTACG2000.043.87364231
GTACGTT2000.043.87364233
CGTATGC1950.043.84479544
TCTCGTA2050.042.80355541
ATGCCGA2050.042.80355522
CTCGTAT2000.042.74867642
GACCGTA2000.042.74867629
TGCCGAG2000.042.74867623
ACGTTAT2100.041.7844235
ACCGTAC2100.041.7844230
TACGTTA2100.041.7844234
CGGAAGA2050.041.7218861
ATCTCGT2050.041.70602840
GCGGTTC2050.041.7060288
CGGTTCA2050.041.7060289
AGACCGT2050.041.70602828
TCGTATG2050.041.70602843
AGAGCGG2150.040.8230325
Produced by FastQC (version 0.11.5)