FastQCFastQC Report
Mon 6 Jun 2016
ERR1379204.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379204.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334903
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATTCTGATCTCGTATGCC13630.40698351462960913Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATTC1850.043.78058631
GACCGAT1850.043.78058629
ACCGATT1850.043.78058630
AGACCGA1850.043.78058628
TCTCGTA1850.042.5644641
TCGTATG1900.041.44434443
CGATTCT2000.040.49704432
GAGACCG2000.040.49704427
ATCTCGT1950.040.38166840
CGTATGC1950.040.38166844
GATCTCG1950.040.38166839
CTCGTAT2000.039.37212442
CGAGACC2150.038.71810526
AGATCGG356.238164E-638.5686117
ATCGGAA356.238164E-638.5686119
AAGAGCG2150.037.694184
GAGCGGT2150.037.677296
GCGGTTC2150.037.671678
CGGAAGA2100.037.5252761
AGAGCGG2200.036.831995
Produced by FastQC (version 0.11.5)