FastQCFastQC Report
Mon 6 Jun 2016
ERR1379192.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379192.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476096
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCGATTATCTCGTATGCC11690.2455387148810324Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA1650.039.5449931
TATCTCG1650.039.5449939
AGACCGT1650.039.5449928
CGTCGAT1650.039.5449932
CGTATGC1700.038.38190544
CTCGTAT1700.038.38190542
TCGTATG1700.038.38190543
ACCGTCG1700.038.38190530
TCTCGTA1750.037.2852841
GCGGTTC1700.037.058398
GTCGATT1850.036.4860633
CGATTAT1850.036.4860635
ATCTCGT1800.036.24957740
GACCGTC1850.035.2698629
CGGTTCA1850.035.269869
GAGACCG1850.035.2698627
TCGATTA1950.034.61498334
CGGAAGA1850.034.060811
AAGAGCG1900.033.160994
GAGCGGT1900.033.157516
Produced by FastQC (version 0.11.5)