FastQCFastQC Report
Mon 6 Jun 2016
ERR1379189.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379189.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219025
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCATTATCTCGTATGCC9870.45063348932770236Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1550.044.99703243
TATCTCG1650.043.63348839
ATCTCGT1600.043.59087440
CGTATGC1600.043.59087444
CGTGCAT1650.042.2699432
CCGTGCA1650.042.2699431
ACCGTGC1650.042.2699430
CGGTTCA1750.041.140149
CTCGTAT1700.041.02670742
TCTCGTA1700.041.02670741
AGACCGT1700.041.02670728
GAGCGGT1800.040.0156336
AGCGGTT1800.040.0064937
GAGACCG1800.039.9973627
GACCGTG1750.039.8545129
GTATGCC1750.039.8545145
AGAGCGG1850.038.934135
GCGGTTC1850.038.925248
CCGAGAC1800.038.7474425
CGGAAGA1750.038.5776941
Produced by FastQC (version 0.11.5)