FastQCFastQC Report
Mon 6 Jun 2016
ERR1379187.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379187.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217844
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACATTATCTCGTATGCC8470.3888103413451828Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC1050.045.00918645
AATGCCG1050.044.9988521
ACCGCAC1100.042.9534530
GAGCGGT1100.042.953456
CGCACAT1100.042.9534532
ATGCCGA1100.042.9534522
CCGCACA1100.042.9534531
GACCGCA1050.042.85605229
AGACCGC1050.042.85605228
CGTATGC1150.041.0953444
CTCGTAT1150.041.0859142
GCGGTTC1150.041.085918
AAGAGCG1150.041.085914
AGCGGTT1150.041.085917
TCTCGTA1150.041.0859141
CGGAAGA1100.040.917441
CCGAGAC1100.040.90804725
TGCCGAG1100.040.90804723
CGGTTCA1200.039.3739939
AGAGCGG1200.039.3739935
Produced by FastQC (version 0.11.5)