FastQCFastQC Report
Mon 6 Jun 2016
ERR1379185.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379185.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426661
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTTGTATCTCGTATGCC13860.3248480643883552Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG1750.042.42724239
CGTATGC1800.041.2487144
CTCGTAT1850.040.1338842
ATCTCGT1900.039.0777340
AGACCGT1900.039.0777328
CGGTTCA2000.038.2488069
AGCGGTT1950.038.0801967
GAGCGGT1950.038.0801966
GACCGTG1950.038.07573329
GAGACCG1950.038.07573327
CCGTGTT2000.037.1238431
TCGTATG2000.037.1238443
TCTCGTA2050.036.2183841
ACCGCAC250.002105949735.9988759
AGAGCGG2100.035.3643265
CCGAGAC2100.035.35603725
CGTGTTG2100.035.35603732
ACCGTGT2100.035.35603730
GCGGTTC2200.034.7757158
CGGAAGA2150.034.5378531
Produced by FastQC (version 0.11.5)