FastQCFastQC Report
Mon 6 Jun 2016
ERR1379183.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379183.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499629
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTGTGTATCTCGTATGCC15040.3010233593326248Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT1850.041.3579756
GACCGTT1900.040.26154729
CGTATGC1950.039.229244
GCGGTTC1950.039.22928
AGCGGTT1950.039.22927
AGACCGT1950.039.229228
AGAGCGG2000.038.2561265
GAGACCG2000.038.2484727
TCTCGTA2000.038.2484741
ACCGTTG2000.038.2484730
CTCGTAT2050.037.3155842
CGTTGTG2050.037.3155832
CGGTTCA2150.036.6264429
AAGAGCG2100.036.4380534
TATCTCG2100.036.42711339
AATGCCG2150.035.5799721
CCGAGAC2150.035.5799725
ATGCCGA2150.035.5799722
CGGAAGA2250.035.0126151
CCGTTGT2200.034.77133631
Produced by FastQC (version 0.11.5)