FastQCFastQC Report
Mon 6 Jun 2016
ERR1379180.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379180.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596368
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCAGTATCTCGTATGCC11540.19350468167306092Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT1450.041.89569528
AGAGCGG1500.040.5025675
TCTCGTA1500.040.4991741
GAGCGGT1550.039.1960336
ATCTCGT1550.039.19274540
CTCGTAT1550.039.19274542
GACCGTT1550.039.19274529
GAGACCG1550.039.19274527
ATGCCGA1550.039.19274522
TGCCGAG1550.039.19274523
CGTATGC1600.037.9679744
AATGCCG1600.037.9679721
GCGGTTC1650.036.817438
TATCTCG1650.036.8174339
CGGAAGA1750.036.0083161
CGTTCAG1700.035.73456232
AGCGGTT1700.035.7345627
CGGTTCA1750.034.7135739
ACCGTTC1750.034.71357330
GTATGCC1850.034.05335645
Produced by FastQC (version 0.11.5)