FastQCFastQC Report
Mon 6 Jun 2016
ERR1379179.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379179.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320138
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTTCAATCTCGTATGCC9720.30361906427852986Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1100.040.90880642
AGAGCGG1250.039.5997285
GCGGTTC1200.039.3747258
CGGTTCA1200.039.3747259
ATCTCGT1150.039.13016540
GACCGTG1150.039.13016529
GAGACCG1150.039.13016527
TCTCGTA1150.039.13016541
AGACCGT1150.039.13016528
GAGCGGT1300.038.0766566
AATCTCG1250.037.7997439
CGTATGC1200.037.49973744
CCGAGAC1250.035.99975225
ACCGTGT1250.035.99975230
CGGAAGA1400.035.362421
AGCGGTT1350.034.999767
TCGTATG1350.033.33310343
CGTGTTC1350.033.33310332
CGAGACC1350.033.33310326
AAGAGCG1500.032.999774
Produced by FastQC (version 0.11.5)