FastQCFastQC Report
Mon 6 Jun 2016
ERR1379177.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379177.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481696
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATCTATCTCGTATGCC9230.19161462831329304Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC1050.040.7131628
CGTATGC1100.038.8625644
GACCGCA1100.038.8625629
TCGTATG1100.038.8625643
ACCGCAA1150.037.17288230
GAGACCG1150.037.17288227
TATCTCG1150.037.17288239
CTCGTAT1200.035.62401642
AATGCCG1200.035.62401621
ATGCCGA1200.035.62401622
CCGCAAT1200.035.62401631
TCTCGTA1250.034.19905541
AGCGCTC401.5589878E-533.7490657
ATCTCGT1300.032.88370540
CGCAATC1300.032.88370532
AGAGCGG1350.031.6723675
CGGTTCA1350.031.665799
TGCCGAG1350.031.6657923
GCGGTTC1400.030.534878
GAGCGGT1400.030.534876
Produced by FastQC (version 0.11.5)