FastQCFastQC Report
Mon 6 Jun 2016
ERR1379172.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379172.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558292
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATTACCATCTCGTATGCC14260.255421893919311Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1700.041.0282742
TCTCGTA1700.041.0282741
GCGGTTC1850.040.137618
GAGCGGT1850.040.137616
ATCTCGT1750.039.85603340
CGTATGC1750.039.85603344
GACCGAT1750.039.85603329
ACCGATT1800.038.7489230
CGATTAC1800.038.7489232
GAGACCG1750.038.57035427
TCGTATG1850.037.70165343
AGAGCGG2000.037.1272935
CGGTTCA1950.036.922059
CCGATTA1900.036.70950331
CCGAGAC1850.036.4854725
AGCGGTT2050.036.221757
AGACCGA1950.035.76823828
AATGCCG2000.034.8740321
ATGCCGA2000.034.8740322
CATCTCG2050.034.0234439
Produced by FastQC (version 0.11.5)