FastQCFastQC Report
Mon 6 Jun 2016
ERR1379164.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379164.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331841
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTCTAAATCTCGTATGCC9500.2862816830952173Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC950.042.6290144
TCTCGTA950.042.6290141
CTCGTAT1000.040.49755542
ACCGCTC1000.040.49755530
TCGTATG1000.040.49755543
CGCTCTA950.040.2607332
AATCTCG1050.038.56910739
GACCGCT1050.036.42637629
CCGAGAC1050.036.42637625
GCGGTTC1150.035.2258838
AGCGGTT1150.035.2258837
ATCTCGT1150.035.21526740
AGACCGC1100.034.7706328
CGAGACC1100.034.7706326
AGAGCGG1200.033.758145
GAGACCG1150.033.2588627
GAGCGGT1250.032.4078146
CGGTTCA1250.032.398059
GTATGCC1250.032.3980545
CGGAAGA1300.031.1566641
Produced by FastQC (version 0.11.5)