FastQCFastQC Report
Mon 6 Jun 2016
ERR1379162.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379162.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364743
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCATATATCTCGTATGCC10490.2875997620242198Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCC1300.041.53833429
AGACCGC1300.041.53833428
TCTCGTA1400.040.1784541
ACCGCCA1350.039.99987830
CGCCATA1350.039.99987832
CTCGTAT1450.038.79298442
CGTATGC1400.038.57131244
GAGACCG1400.038.57131227
TATCTCG1400.038.57131239
TCGTATG1400.038.57131243
CCGCCAT1400.038.57131231
GCGGTTC1500.037.4998868
CCGAGAC1450.037.24126425
AAGAGCG1550.036.290214
CGGTTCA1550.036.290219
AGCGGTT1550.036.290217
GAGCGGT1550.036.290216
AGAGCGG1550.036.290215
ATCTCGT1600.035.15614340
AATGCCG1600.035.15614321
Produced by FastQC (version 0.11.5)