FastQCFastQC Report
Mon 6 Jun 2016
ERR1379161.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379161.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377475
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTTGATATCTCGTATGCC8580.22729982118021064Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCT950.042.62994829
GAGACCG950.042.62994827
TATCTCG1050.040.7127339
AAGAGCG1200.039.3891454
ATCTCGT1100.038.86215240
CGTATGC1100.038.86215244
CTCGTAT1100.038.86215242
TCTCGTA1100.038.86215241
AGAGCGG1200.037.5035325
GCGGTTC1200.037.4985668
TCGTATG1150.037.1724943
AGACCGC1100.036.81677628
CGGAAGA1250.036.0129361
GAGCGGT1250.035.9986236
CGGTTCA1200.035.623649
AGCGGTT1300.034.6140637
ACCGCTT1250.034.19869230
CCGAGAC1250.032.3987625
CCGCTTG1350.031.66545531
AATGCCG1350.031.66545521
Produced by FastQC (version 0.11.5)