FastQCFastQC Report
Mon 6 Jun 2016
ERR1379159.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379159.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407879
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTTCAAATCTCGTATGCC7290.17872947614366025Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC850.044.9990228
TCTCGTA900.044.99901641
ATCTCGT1000.042.7490740
CTCGTAT950.042.63065342
GACCGCT900.042.49907329
CGTATGC950.040.26228344
GAGACCG950.040.26228327
TCGTATG950.040.26228343
CGCTTCA1050.038.57058732
ACCGCTT1000.038.24916530
GTATGCC1000.038.24916545
AGAGCGG1000.038.2491655
GCGGTTC1050.036.4277768
CGGTTCA1100.034.771979
AATGCCG1100.034.7719721
CCGAGAC1100.034.7719725
AATCTCG1200.033.74926439
AAGAGCG1150.033.2683034
CGAGACC1250.032.39929226
ATGCCGA1200.031.87430422
Produced by FastQC (version 0.11.5)