FastQCFastQC Report
Mon 6 Jun 2016
ERR1379158.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379158.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428063
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTCAAATCTCGTATGCC10600.24762710161821974Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACCCG207.02841E-444.99988635
CTCGTAT1200.043.12488642
AGACCGT1200.043.12488628
TCGTATG1200.043.12488643
GACCGTC1150.043.04336529
TCTCGTA1300.039.80758741
CGTATGC1350.038.33323744
GCGGTTC1350.038.3332378
ACCGTCT1300.038.07682430
ATCTCGT1400.036.9641940
CGGTTCA1400.036.964199
GAGCGGT1400.036.964196
AATCTCG1400.036.9641939
AGCGGTT1450.035.689567
GAGACCG1450.035.6895627
CCGAGAC1450.035.6895625
AGAGCGG1450.035.689565
CGGAAGA1450.034.141831
AAGAGCG1550.033.387014
TGCCGAG1700.033.0881523
Produced by FastQC (version 0.11.5)