FastQCFastQC Report
Mon 6 Jun 2016
ERR1379157.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379157.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530830
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTGCATATCTCGTATGCC9980.1880074600154475Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTT900.042.49599529
CTCGTAT950.040.25936542
TATCTCG950.040.25936539
TCTCGTA950.040.25936541
TCGTATG950.040.25936543
CCGTTGC950.040.25936531
CGTATGC1000.038.24639544
AGACCGT1000.038.24639528
GCGGTTC950.037.8983088
CGGTTCA950.037.891179
ACCGTTG1050.036.4251430
GAGCGGT1000.036.0101786
AGCGGTT1000.036.003397
CGTTGCA1150.035.2140732
ATCTCGT1100.034.7694540
TGCCGAG1100.034.7694523
AAGAGCG1050.034.3018724
CCGAGAC1150.033.25773225
ATGCCGA1150.033.25773222
AGAGCGG1100.032.739615
Produced by FastQC (version 0.11.5)