FastQCFastQC Report
Mon 6 Jun 2016
ERR1379152.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379152.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613817
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGTTAATCTCGTATGCC10780.17562237605019085Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTC1350.041.6676458
TCGTATG1250.041.39760643
AATGCCG1350.039.9976821
CTCGTAT1300.039.80538642
CGAAGTT1300.039.80538632
GAGCGGT1450.038.80356
AGAGCGG1450.038.80355
CGGTTCA1450.038.7908559
CCGAAGT1400.038.5691931
ATGCCGA1400.038.5691922
GACCGAA1350.038.33111229
TCTCGTA1350.038.33111241
CGTATGC1450.037.23922344
GAGACCG1400.036.96214327
AGACCGA1400.036.96214328
CCGAGAC1400.036.96214325
TGCCGAG1400.036.96214323
CGGAAGA1550.036.3059651
AGCGGTT1550.036.2911767
ACCGAAG1550.034.83669330
Produced by FastQC (version 0.11.5)