FastQCFastQC Report
Mon 6 Jun 2016
ERR1379146.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379146.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250137
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAATGAATCTCGTATGCC15790.6312540727681231Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2150.043.95270542
AGACCGT2100.043.92778828
CGTATGC2200.042.9537844
GACCGTA2150.042.90621629
GTATGCC2250.041.99925245
TCGTATG2250.041.99925243
CCGTAAT2200.041.93107231
ATCTCGT2300.041.08622740
TCTCGTA2300.041.08622741
CGTAATG2300.041.08622732
GAGACCG2250.040.9992727
CCGAGAC2250.040.9992725
GCGGTTC2350.040.212058
ACCGTAA2300.040.10798330
CGGTTCA2400.039.37439
GAGCGGT2400.039.37436
AATCTCG2400.039.374339
CGAGACC2400.039.374326
AGCGGTT2500.038.6993147
GCCGAGA2450.038.57074424
Produced by FastQC (version 0.11.5)