FastQCFastQC Report
Mon 6 Jun 2016
ERR1379145.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379145.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517019
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAAGAATCTCGTATGCC50800.3348672627040268Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAAGAATCTCGTATGCCGTC47910.3158167432312977Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6450.041.85534743
ATGCCGT7250.036.92651744
TGCCGTC7850.034.10410745
CTCGTAT11500.023.47538839
ACCGTTA11600.023.46618327
CGTTAAG11650.023.3662429
CCGTTAA11750.023.35884328
GACCGTT11750.023.16661526
CGGAAGA9350.023.1300371
CGTATGC11850.022.97187441
AGACCGT12250.022.58832725
TCGTATG12050.022.40389840
GTATGCC12400.022.31581742
TCTCGTA12200.022.12771238
ATCTCGT12500.021.5980737
AATCTCG12650.021.51981736
GAGACCG12800.021.26622824
CCGAGAC13200.020.79222722
GCGGTTC14950.020.031445
CGGTTCA14850.020.0140466
Produced by FastQC (version 0.11.5)