FastQCFastQC Report
Mon 6 Jun 2016
ERR1379143.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379143.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1565319
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGACATAAATCTCGTATGCCGTC26090.16667529110679677Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACATAAATCTCGTATGCC23470.14993748878024224Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3900.035.1890143
ATGCCGT4650.028.54571244
TGCCGTC5450.025.18112645
CGGAAGA6700.020.8449151
TCGTATG6700.020.48315640
CTCGTAT6750.020.33142939
CGTATGC6900.020.21549641
CGACATA6950.019.74761432
TCTCGTA7250.019.54739438
GACCGAC7650.017.93892129
CCGACAT8100.017.77724631
AGACCGA8050.017.60535828
GAGCGGT9600.017.5944653
ACCGACA8000.017.43530830
AGCGGTT9700.017.4130784
GAGACCG8300.017.34555427
AGAGCGG10100.017.1754532
CGGTTCA9750.017.0906126
ATCTCGT8500.016.93958937
CCGAGAC8150.016.83676122
Produced by FastQC (version 0.11.5)