FastQCFastQC Report
Mon 6 Jun 2016
ERR1379140.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379140.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264072
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTAAGATATCTCGTATGCCGTC56790.4492623837882652Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAAGATATCTCGTATGCC45400.35915675689359466Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6200.040.64176643
ATGCCGT7150.034.92715544
TGCCGTC7400.033.74717745
CGGAAGA9000.023.274761
TCTCGTA11000.022.90355338
CCGTAAG11100.022.90258828
CGTAAGA11200.022.89987229
GACCGTA11100.022.8998726
ACCGTAA11100.022.8998727
CTCGTAT11050.022.80352439
TCGTATA1104.0654413E-922.4945629
CGTATGC11350.022.2007941
TCGTATG11350.022.2007940
AGACCGT11750.022.01682525
GTATGCC11800.021.5448142
TATCTCG11700.021.53751436
CGTATAA1156.7902874E-921.51653710
ATCTCGT11750.021.44586637
GAGACCG12300.020.84944324
AGCGGTT14450.020.2589594
Produced by FastQC (version 0.11.5)