FastQCFastQC Report
Mon 6 Jun 2016
ERR1379137.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379137.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1409226
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATTAAGATCTCGTATGCC41340.2933525211711961Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATTAAGATCTCGTATGCCGTC38670.2744059505004875Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5200.036.34153743
ATGCCGT5300.035.6558544
TGCCGTC6150.030.72780845
CGGAAGA7250.026.4040681
CGATTAA9100.021.263332
CTCGTAT9000.021.24730339
ACCGATT9150.021.14560530
CCGATTA9300.020.80602531
TCTCGTA9400.020.34243838
TCGTATG9700.020.17784943
GACCGAT9850.019.87127729
CGTATGC9650.019.81613744
AGACCGA9900.019.31504428
ATCTCGT10250.018.8756540
GAGACCG10250.018.65550427
GTATGCC10300.018.56560345
AATGCCG12000.018.37201518
CCGAGAC10600.018.25304825
GAAGAGC11000.018.0130543
AGCGGTT12400.017.7951554
Produced by FastQC (version 0.11.5)