FastQCFastQC Report
Mon 6 Jun 2016
ERR1379136.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379136.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729701
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTAATATCTCGTATGCC31130.42661309221174154Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTAATATCTCGTATGCCGTC30250.4145533581562859Illumina Paired End PCR Primer 2 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4000.037.6833743
ATGCCGT4400.033.23499344
TGCCGTC4650.031.44816445
CGGTAAC300.00514880129.9967144
CGGAAGA5450.023.563441
CACTAGG602.470433E-422.4944518
TCTCGTA6650.022.3253238
TCGTATG6750.021.99758740
CGAGTAA6900.021.84393529
CTCGTAT6800.021.8358439
ACCGAGT6800.021.83284827
CGTATGC7000.021.53335241
GTATGCC7000.021.53335242
CCGAGTA6950.021.363128
TATCTCG7150.021.08160436
AGACCGA7550.019.66402225
GAGCGGT9800.019.5372663
AGCGGTT9900.019.339924
GACCGAG7700.019.28095626
AATGCCG9450.019.28095618
Produced by FastQC (version 0.11.5)