FastQCFastQC Report
Mon 6 Jun 2016
ERR1379135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827388
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAATAACATCTCGTATGCC32030.3871218823574913Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAATAACATCTCGTATGCCGTC29620.3579940729137962Illumina Paired End PCR Primer 2 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3650.032.6726843
ATGCCGT3950.030.19121244
TGCCGTC4250.028.06006845
CGGAAGA5000.025.6759741
TCGTATG6500.019.73164243
CTCGTAT6550.019.5810242
CGAATAA6700.019.47965432
CCGAATA6700.019.47965431
CGTATGC6600.019.4326844
TCTCGTA6650.019.2865741
AGCGGTT8200.019.2197884
CGGTTCA8500.019.068896
GAGCGGT8300.018.9882243
GCGGTTC8400.018.7610385
GTATGCC6850.018.7234645
AGAGCGG8450.018.6601852
GAATAAC7150.018.56837833
ACCGAAT7150.018.56388930
GACCGAA7300.018.49061829
ACTAGAC2600.018.1683919
Produced by FastQC (version 0.11.5)