FastQCFastQC Report
Mon 6 Jun 2016
ERR1379133.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379133.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026934
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGTGATCTCGTATGCC31830.30995175931462Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGTGATCTCGTATGCCGTC30320.29524779586614136Illumina Paired End PCR Primer 2 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3400.037.0515343
ATGCCGT3850.033.30512644
TGCCGTC4150.030.35546745
CGGAAGA7050.023.3353251
CTCGTAT7000.023.13830242
TCTCGTA6950.022.98108741
TCGTATG7050.022.97419743
CGAGTGT7300.022.4999532
CGTATGC7250.022.34042744
ACCGAGT7400.022.19157430
AGACCGA7750.021.18937528
CCGAGTG7800.021.05764831
GACCGAG7800.021.05354529
GAGACCG8100.020.82821727
ATCTCGT7700.020.74266640
GTATGCC7900.020.21753745
CCGAGAC8350.020.20658525
GAAGAGC8850.019.5953163
CGAGACC9450.018.56686826
AGCGGTT9600.018.2776497
Produced by FastQC (version 0.11.5)