FastQCFastQC Report
Mon 6 Jun 2016
ERR1379131.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379131.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846894
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGAGTCATCTCGTATGCCGTC28710.33900346442411916Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGAGTCATCTCGTATGCC26770.31609622928016967Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3700.035.26823443
ATGCCGT4350.030.5154844
TGCCGTC4700.027.76435545
CGGAAGA4250.024.9162271
TCTCGTA5800.022.88525838
CTCGTAT5800.022.49870139
CGAGAGT5900.021.7347529
CGTATGC6150.021.5841241
TCGTATG6100.021.39220640
GAGCGGT7750.021.2123683
CCGAGAG6100.021.02213528
GTATGCC6350.020.90430542
ACCGAGA6200.020.68184727
CGGTTCA7850.020.3659826
AGCGGTT8050.020.1420944
GCGGTTC8050.020.1409025
AGAGCGG8300.019.8114132
AAGAGCG8200.019.7829951
GAGAGTC6800.019.18893430
CCGAGAC6800.019.18780122
Produced by FastQC (version 0.11.5)