FastQCFastQC Report
Mon 6 Jun 2016
ERR1379130.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379130.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314992
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAGTAATCTCGTATGCCGTC34290.26076204265881464Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAGTAATCTCGTATGCC28320.21536252692031585Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4850.036.18422343
GCGTTAG250.002108389735.99592613
TGCCGTC5050.035.19671245
ATGCCGT5400.032.91544344
CGGAAGA6550.022.3495121
CGTATGC8000.021.93668641
CTCGTAT8150.021.80900439
TCGTATG8200.021.40164440
CGGCAGT8450.021.29936429
TCTCGTA8500.021.17407438
AGACCGG8650.021.06697825
GTATGCC8450.020.7684642
ATCTCGT8900.020.72877737
GAGACCG8900.020.47520824
AGCGGTT11550.020.269784
GAGCGGT12050.019.4287093
CGGTTCA12000.019.3205976
CCGAGAC9550.019.31718422
GCGGTTC12050.019.240435
AGAGCGG12400.019.0669362
Produced by FastQC (version 0.11.5)