FastQCFastQC Report
Mon 6 Jun 2016
ERR1379128.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379128.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598859
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGATGATCTCGTATGCCGTC66681.1134507455010278Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGATGATCTCGTATGCC59320.9905503632741597Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT15010.25064330668821877Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC10580.1766692994511229Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7800.040.6676443
ATGCCGT8000.039.3697444
TGCCGTC8450.037.2731345
CCGTCCC300.00514867529.99599341
CGGAAGA11200.026.7531571
GAAGAGC12200.024.5438563
GGAAGAG13500.023.0276932
CTCGTAT14400.022.18453439
CGTATGC14550.021.9558341
TCTCGTA14550.021.9558338
TCGTATG14650.021.8059640
ACCGGTG14950.021.51706927
GACCGGT14850.021.5104826
GTATGCC14900.021.44008842
CCGGTGA15200.021.16670228
AGACCGG15200.021.01692825
AGAGCGG20200.020.7427652
ATCTCGT15350.020.66671837
GAGACCG15350.020.66499124
GAGCGGT20350.020.5778263
Produced by FastQC (version 0.11.5)