FastQCFastQC Report
Mon 6 Jun 2016
ERR1379126.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379126.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908465
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCGAATCTCGTATGCCGTC18230.20066816002817942Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCGAATCTCGTATGCC16800.18492732246151475Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2150.030.34460443
ATGCCGT2550.025.58466744
TGCCGTC2850.022.89154445
CGAATCT3900.021.92243437
CTCGTAT3800.020.72079542
CGGAAGA4250.020.668071
TCGTATG3800.020.12877343
TCGAATC4200.019.82084736
TCTCGTA4000.019.68475341
ACCGGAT4250.019.58658230
GACCGGA4250.019.58658229
CGTATGC4050.018.88625544
ATCTCGT4550.018.29415140
GATCGAA4800.017.34419634
GTATGCC4550.017.30527945
AATCTCG4550.017.30527939
AGACCGG5150.016.16368528
GAGACCG5150.016.16190527
GGATCGA5250.015.8575533
CCGGATC5350.015.56114731
Produced by FastQC (version 0.11.5)