FastQCFastQC Report
Mon 6 Jun 2016
ERR1379124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819412
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCGTAGAATCTCGTATGCCGTC36830.4494686433686595Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCGTAGAATCTCGTATGCC36780.44885844971760236Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4650.037.73502743
TGCCGTC4850.036.1789445
ATGCCGT5100.034.40546444
CCGCGTA8450.022.63175631
CTCGTAT8500.022.49588242
TCGTATG8500.022.49588243
TGAACCG702.7055943E-522.4958810
CGGAAGA8950.022.3989091
TCTCGTA8650.022.1057841
CGTATGC8750.021.85314244
CGTAGAA8850.021.6088534
CGCGTAG8750.021.5986832
GCGTAGA8900.021.48745333
GTATGCC9350.020.69139745
ACCGCGT9300.020.56200830
AGACCGC9500.020.12789328
ATCTCGT9550.020.02251240
GAGACCG9650.019.81502327
AATCTCG9700.019.71288539
TCTTCCG807.570261E-519.68389518
Produced by FastQC (version 0.11.5)