FastQCFastQC Report
Mon 6 Jun 2016
ERR1379120.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379120.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841186
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGAGTATCTCGTATGCC47940.5699096275972257Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGAGTATCTCGTATGCCGTC40500.4814630771315737Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGTA207.0380204E-444.99245540
TATGCCG5300.041.17233743
ATGCCGT6300.034.9941344
TGCCGTC6500.033.91738545
CGGAAGA7650.025.6159211
TCGTATG9450.023.3294240
CTCGTAT9500.022.9698339
CGTATGC9600.022.96489741
TCTCGTA9700.022.72814638
GTATGCC9650.022.61278742
AGACCGC10000.022.04630325
ATCTCGT10150.021.94343837
TATCTCG9900.021.5885736
GACCGCA10300.021.40417526
CGCAGAG10300.021.40417529
CCGCAGA10550.021.110228
GAGTATC10150.021.05683333
ACCGCAG10500.020.99647727
GAAGAGC9950.020.5964743
CCGAGAC11050.020.1549922
Produced by FastQC (version 0.11.5)