FastQCFastQC Report
Mon 6 Jun 2016
ERR1379118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190987
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTCTCTATCTCGTATGCC32570.2734706592095464Illumina Paired End PCR Primer 2 (97% over 38bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCTCTCTATCTCGTATGCCGTC32000.26868471276344746Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4150.034.6976343
ATGCCGT4650.031.45055444
TGCCGTC5000.029.2490245
CGGAAGA5950.025.358711
CGTATGC7250.020.79240644
TCGTATG7350.020.20340343
CTCGTAT7300.020.03357542
GACCGCT7450.019.9313829
TCTCGTA7350.019.89729141
TATCTCG7500.019.79933539
AGACCGC7600.019.53881825
ATCTCGT7700.018.99366837
CCGCTCT7950.018.67783428
GTATGCC8300.018.43311545
ACCGCTC8000.018.27986730
GCGGTTC9750.018.2362825
GAGCGGT9900.018.189613
CGCTCTC8050.018.16785432
CGGTTCA9800.018.143246
GAAGAGC8600.018.060063
Produced by FastQC (version 0.11.5)