FastQCFastQC Report
Mon 6 Jun 2016
ERR1379117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138172
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTAGCAATCTCGTATGCCGTC32510.28563345434609183Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTAGCAATCTCGTATGCC32460.2851941534319945Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4300.037.67085343
ATGCCGT5100.031.761744
TGCCGTC5400.029.99715845
CGGAAGA7150.024.5679551
ACCGGTA8250.022.08881830
CGTATGC8250.022.08881844
GACCGGT8250.022.08881829
CTCGTAT8500.021.96850842
TCTCGTA8400.021.96220641
CCGGTAG8300.021.95671731
CGGTAGC8450.021.56695232
TCGTATG8700.021.46348443
AGACCGG8900.020.72837428
GTATGCC8800.020.70826745
GAGACCG9100.020.51913327
ATCTCGT9700.019.25075540
GGTAGCA9550.019.08280233
AATCTCG9700.019.01881839
GTAGCAA9950.018.7678934
CTAGACG600.00651401918.74822420
Produced by FastQC (version 0.11.5)