FastQCFastQC Report
Mon 6 Jun 2016
ERR1379116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000484
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATAGCGATCTCGTATGCC42840.4281927547067219Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATAGCGATCTCGTATGCCGTC42350.42329512515942286Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6000.037.86982343
ATGCCGT6400.035.85447744
TGCCGTC6650.033.82996445
CGGAAGA8750.024.2007851
CTCGTAT10450.021.31287839
CCGATAG10700.021.02516428
ATAGCGA10600.021.01338231
TAGCGAT10650.020.91472632
CGTATGC10650.020.91263641
TCTCGTA10650.020.9115938
GATAGCG10800.020.83048630
GTATGCC10700.020.81491342
ACCGATA10700.020.81387327
TCGTATG10800.020.62218340
CGATAGC11000.020.45175229
GACCGAT11200.019.88468226
GTGACGT807.5696924E-519.684818
GAAGAGC10900.019.6250693
ATCTCGT11450.019.45343437
AGCGATC11500.019.36885633
Produced by FastQC (version 0.11.5)