FastQCFastQC Report
Mon 6 Jun 2016
ERR1379115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119971
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGATACGATCTCGTATGCCGTC35270.3149188684349863Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATACGATCTCGTATGCC32050.2861681239960678Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4050.034.99541543
ATGCCGT4350.032.5819444
TGCCGTC4450.031.84976245
CGGAAGA7100.023.7953031
CGGATAC7500.022.19907632
CCGGATA7750.022.06359731
CTCGTAT7550.021.7521242
TCTCGTA7550.021.7521241
ACCGGAT7700.021.6215130
CGTATGC7500.021.59717244
GGATACG7850.021.4959233
TCGTATG7550.021.45414443
ATACGAT7800.021.34526435
GATACGA7850.021.20930934
AGACCGG7900.021.07318928
TACGATC8000.020.80977436
GACCGGA7900.020.78934529
ACGGGTT550.003940481720.45095413
ATCTCGT8200.020.3022240
GTATGCC8100.020.27512245
Produced by FastQC (version 0.11.5)