FastQCFastQC Report
Mon 6 Jun 2016
ERR1379113.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379113.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences703883
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGACAATCTCGTATGCCGTC36620.5202569176979697Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGACAATCTCGTATGCC31280.44439203674474304Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT8090.11493387395348374Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4200.039.6373543
ATGCCGT4800.034.68268244
TGCCGTC5100.032.64252545
CGGAAGA6650.024.7221641
GAAGAGC7950.022.3792323
TCGTATG7900.021.64256143
CTCGTAT8000.021.3720342
TCTCGTA8000.021.3720341
CGCAGAC8050.021.2438132
GTATGCC8250.020.72439245
CGTATGC8300.020.59954844
ACCGCAG8200.020.57494530
CCGCAGA8400.020.09077631
AGACCGC8500.019.84877225
GACCGCA8550.019.46959526
ATCTCGT8800.019.42911740
GAGACCG8700.019.39247927
GGAAGAG9500.018.9676462
GACAATC9050.018.89643133
AATCTCG9100.018.78859739
Produced by FastQC (version 0.11.5)