FastQCFastQC Report
Mon 6 Jun 2016
ERR1379110.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379110.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1266031
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGACTAGATCTCGTATGCC21530.1700590269906503Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGACTAGATCTCGTATGCCGTC19860.15686819675031655Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2400.034.68439543
ACACGGG300.00515169729.99494420
AAACGCG300.00515368729.9925759
ATGCCGT3050.027.29263744
TGCCGTC3450.024.12827545
CGGACTA4350.021.72648232
CTCGTAT4500.020.9981242
TCGTATG4650.020.80458843
CGTATGC4600.020.54163744
TCTCGTA4650.020.32075941
CCGGACT4800.019.68962731
CGGAAGA5500.018.8403111
GTACCGC600.00652016318.7453618
ACCGGAC5050.018.71045730
AGACCGG5050.018.71045728
CCGAGAC5350.018.08106425
GGACTAG5250.018.00194433
GACCGGA5250.017.99767729
GTATGCC5500.017.58933445
ATCTCGT5550.017.4308740
Produced by FastQC (version 0.11.5)