FastQCFastQC Report
Mon 6 Jun 2016
ERR1379105.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379105.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153053
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCATGAGATCTCGTATGCCGTC33250.288364888691153Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCATGAGATCTCGTATGCC32340.28047279700065825Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3800.036.70405243
ATGCCGT4200.033.20842744
TGCCGTC4450.031.84831645
CGTATGC5900.024.02118741
TCGTATG5900.023.63989840
GTATGCC6150.023.04471642
CTCGTAT6100.022.8648239
TCTCGTA6150.022.67892638
AGACCGC6350.022.31986425
GACCGCA6600.021.47348426
ATCTCGT6600.021.13263537
ACCGCAT6600.021.13263527
CCGCATG6800.020.84281528
GAGACCG6950.020.06927324
CGCATGA7300.019.10704629
CGGAAGA6750.019.0271781
AATGCCG8700.018.61740718
CCGAGAC7450.018.42036822
AGCGGTT9500.018.0155744
CGGTTCA9450.017.8718196
Produced by FastQC (version 0.11.5)