FastQCFastQC Report
Mon 6 Jun 2016
ERR1379104.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379104.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences728843
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAGATATCTCGTATGCCGTC40900.5611633781212141Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAGATATCTCGTATGCC34610.47486221312408844Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT11880.16299806679902257Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4850.040.81907743
ATGCCGT5400.036.6615844
GTTCGTA250.002098118736.0296133
TGCCGTC5850.033.84145745
CATCCGT300.00515125729.9937815
CGGAAGA7600.022.8226411
CTCGTAT8600.022.7584739
CGTATGC8750.022.62543141
TCGTATG8800.022.49687840
TCTCGTA8750.022.3667938
GTATGCC8950.022.11983742
TATCTCG8850.022.11709236
AATGCCG12300.021.76377918
AGACCGG9200.021.51727725
ATCTCGT9150.021.39194337
TGCCGAG12900.021.10027520
GAAGAGC8350.021.0352543
ATGCCGA12950.021.01880619
GCAGATA9800.020.89282631
AGCGGTT12900.020.7728884
Produced by FastQC (version 0.11.5)