FastQCFastQC Report
Mon 6 Jun 2016
ERR1379101.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379101.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023918
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGACAGATCTCGTATGCC28160.27502202324795544Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGACAGATCTCGTATGCCGTC26580.2595911000685602Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3000.035.24514443
ATGCCGT3450.030.6479544
TGCCGTC3850.027.46374745
CGGAAGA6200.023.2453121
TCGTATG6150.022.67980243
TCTCGTA6050.022.31097441
CTCGTAT6100.022.128142
GACCGTG6650.021.65432429
CGTATGC6250.021.59702344
AGACCGT6700.021.156928
CGTGACA6600.021.13757932
ATCTCGT6450.020.92734740
GTATGCC6450.020.92734745
CCGTGAC6700.020.82209231
GAGACCG6850.020.36116227
CCGAGAC7400.019.15370225
ACCGTGA7300.019.10975530
AGATCTC8050.017.6054138
GAAGAGC8300.017.0884763
GAGCGGT8350.016.986156
Produced by FastQC (version 0.11.5)