FastQCFastQC Report
Mon 6 Jun 2016
ERR1379100.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379100.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704863
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGACACATCTCGTATGCC38720.5493266067306697Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGACACATCTCGTATGCCGTC36300.5149936938100028Illumina Paired End PCR Primer 2 (96% over 31bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT8300.11775337902542764Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5150.036.6946943
ATGCCGT5100.036.6133244
TGCCGTC5750.032.47442245
TGAACCG651.5248972E-524.22618510
CGGAAGA7300.024.0741181
GTATGCC8600.021.7125542
CTCGTAT8750.021.59744839
TCTCGTA8800.021.4732138
CGTATGC8750.021.34033641
AACCGCT754.6061003E-520.99602712
TCGTATG8900.020.98066540
CGAGACA9250.020.91644529
GAAGAGC8400.020.909673
AGACCGA9300.020.56062725
ACCGAGA9450.020.47231727
GACCGAG9500.020.12777126
ATCTCGT9450.019.99905437
GAGACCG9700.019.71276524
CATCTCG9800.019.51438336
CGGTTCA12350.019.1422316
Produced by FastQC (version 0.11.5)