FastQCFastQC Report
Mon 6 Jun 2016
ERR1379098.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379098.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054612
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCACTAATCTCGTATGCCGTC40790.3867773171555036Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCACTAATCTCGTATGCC36370.3448661687900384Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT10780.10221768764246947Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT5150.038.44101344
TATGCCG5300.037.77752343
TGCCGTC5850.034.22579245
CGGAAGA8050.023.2317351
CGTATGC9100.022.00229541
CTCGTAT9050.021.87527339
TCGTATG9250.021.645540
TCTCGTA9250.021.40026338
CGTCACT9450.021.18839829
GACCGTC9600.021.08868426
ATCTCGT9500.020.84105137
ACCGTCA9650.020.74631127
GTATGCC9750.020.53547542
AGACCGT9850.020.32795725
AATCTCG9750.020.30666536
AATGCCG12850.020.30640418
CCGTCAC9950.020.12365528
TGCCGAG13350.019.54864720
GAGACCG10250.019.5346724
GTCACTA10250.019.5346730
Produced by FastQC (version 0.11.5)