FastQCFastQC Report
Mon 6 Jun 2016
ERR1379097.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379097.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2034642
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTATCATCTCGTATGCCGTC39850.19585755135301444Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTATCATCTCGTATGCC32290.1587011375957048Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4850.036.18210643
TGCCGTC5050.035.19465645
ATGCCGT5100.034.8496144
CGGAAGA8250.021.3036821
CTCGTAT8850.020.3375439
CCGTGTA8650.020.28857431
TCTCGTA8850.020.08085338
CGTATGC8850.019.82861341
TCGTATG9100.019.28434640
CGTGTAT9100.019.03804632
AGACCGT9400.018.66705328
ATCTCGT9550.018.37610837
GACCGTG9600.018.27815429
GAGACCG9850.018.04218724
AGCGGTT12150.017.9789224
GTATGCC9950.017.63650542
ACCGTGT9950.017.63520430
GAGCGGT12400.017.6164443
CGGTTCA12400.017.613416
AATGCCG12150.017.58831818
Produced by FastQC (version 0.11.5)