FastQCFastQC Report
Mon 6 Jun 2016
ERR1379095.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379095.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1265406
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTATCTATCTCGTATGCC35820.28307120402463715Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTATCTATCTCGTATGCCGTC32640.2579409296304901Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3650.035.7510443
TGCCGTC4350.031.03241245
ATGCCGT4750.028.41915544
CGGAAGA7700.026.6291281
ACCGGTA7600.026.04780630
CTCGTAT7700.025.7125742
CCGGTAT7800.025.3859331
CGGTATC7950.025.18998532
CGTATGC8000.025.02957744
TCTCGTA8050.024.59463141
TCGTATG8100.024.44281243
GACCGGT8150.024.28997829
TATCTCG8600.023.02171939
AGACCGG8750.022.8805728
GTATGCC8850.022.8798345
CCGAGAC8700.022.75350425
CGGCTAG500.002268216822.49405317
ATCTCGT9050.021.87699340
GAGACCG9050.021.87267127
ATCTATC9750.020.53790336
Produced by FastQC (version 0.11.5)