FastQCFastQC Report
Mon 6 Jun 2016
ERR1379094.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379094.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665225
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGATCTATCTCGTATGCCGTC34790.5229809462963659Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGATCTATCTCGTATGCC32130.4829944755533842Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT6820.10252170318313353Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3950.037.0253843
ATGCCGT4400.033.23869344
TGCCGTC5300.028.01891345
TCTCGTA6450.022.66934438
CTCGTAT6500.022.50003839
ACCCGAC500.002264581622.49834637
GTATGCC6550.022.32828142
TCGTATG6600.022.15912640
CGTATGC6700.021.82839641
CGGAAGA6150.021.606521
CCGTGAT7000.021.21113228
ATCTCGT6900.021.19409437
TATCTCG7000.020.89132136
AGACCGT7000.020.88818225
ACCGTGA7200.020.62038227
CACGAGC550.003917676420.469975
GACCGTG7200.020.30795326
GAAGAGC7050.019.8051363
ATCTATC7700.019.28429633
CGGTTCA9700.018.8028286
Produced by FastQC (version 0.11.5)